【文獻標題】Identification, characterization, and immunological analysis of complement component 4 from grass carp (Ctenopharyngodon idella)

【作者】Xin-Zhan Meng，Shen-Tong Wang，Xiao-Yan Xu，et.al

【作者單位】上海海洋大學（Shanghai Ocean University）

【文獻中引用產(chǎn)品】

魚肌酸激酶(CK)ELISA試劑盒

【關鍵詞】Complement component 4，Primary liver cells，Innate immune response，Aeromonas hydrophila，Grass carp

【DOI】doi.org/10.1016/j.fsi.2020.06.043

【影響因子(IF)】5.50

【出版期刊】《Fish and Shellfish Immunology》

【產(chǎn)品原文引用】

Whole-blood samples were allowed to clot at 4℃ overnight and then centrifuged (1000×g, 4℃ ) for 20 min. Then, equal volumes of supernatant (serum) samples from each control group were mixed, while samples of each experimental group with equal volumes at the same time point were mixed, and then stored at -80℃. Following method of Li et al. [24], the complement C4 contents in fish serum were determined using kits from Hengyuan Ltd., Shanghai, China, according to the  manufacturer’s instructions.