The CD226:CD112 Homogeneous Assay Kit is designed to measure the inhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.