Recombinant human EZH2 (Histone-lysine N-methyltransferase) in complex with EED (Embryonic ectoderm development protein) and SUZ (Suppressor of zeste).

EZH2, full length encompassing amino acids 2-751 (end), contains an N-terminal His-tag (6xHis). Polycomb protein EED, full length encompassing amino acids 2-441(end), contains an N-terminal FLAG-tag. Polycomb protein SUZ12 full length encompassing amino acids 2-739 (end), also contains an N-terminal His-tag (6xHis). The complex was affinity purified using the FLAG-tag of EED.

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