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BPS Bioscience Inc.
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當前位置:BPS Bioscience Inc.>>PARP Assay Kits>> 78814Chemi-Verse™ PARP7 Assay Kit

Chemi-Verse™ PARP7 Assay Kit

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產品型號78814

品       牌BPS Bioscience

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更新時間:2024-11-27 07:02:44瀏覽次數:268次

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●Purchase●DescriptionTheChemi-Verse™PARP7AssayKitisanELISA-typechemiluminescentassaydesignedtomeasurePARP7enzymaticactivityforscreeningandprofilingapplications
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Description

The Chemi-Verse™ PARP7 Assay Kit is an ELISA-type chemiluminescent assay designed to measure PARP7 enzymatic activity for screening and profiling applications. PARP7 catalyzes the NAD-dependent ADP-ribosylation of histones. This 96-well or 384-well format assay kit contains sufficient amounts of purified PARP7 enzyme (amino-acids 400-657), histone mixture, and PARP assay buffer for 100 or 400 enzyme reactions. The Chemi-Verse™ PARP7 Assay Kit takes advantage of a highly specific ADP-ribose binding reagent. First, histone proteins are coated on a 96-well or 384-well plate. Next, an NAD+ substrate is incubated with the PARP7 enzyme in an optimized assay buffer. Finally, the plate is treated with the ADP-ribose specific binding reagent and secondary HRP-conjugated antibody, followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Figure 1. Chemi-Verse™ PARP7 chemiluminescent assay schematic.

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