細胞名稱

人結腸癌細胞甲氨蝶呤耐藥株HT29 MTX

貨物編碼

BFN60803898

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研           3類

培養體系

DMEM高糖培養基+10%FBS+1%雙抗 

培養溫度

37℃

二氧化碳濃度

5%

簡介

人結腸癌細胞甲氨蝶呤耐藥株HT29 MTX，野生株取自44歲女性，該細胞耐藥倍數15倍，IC50 2100ng/ml

注釋

Heterozygous for APC p.Glu853Ter (c.2557G>T) and p.Thr1556fs*3 (c.4666_4667insA) (from parent cell line).

Heterozygous for BRAF p.Val600Glu (c.1799T>A) (from parent cell line).

Heterozygous for PIK3CA p.Pro449Thr (c.1345C>A) (from parent cell line).

Homozygous for SMAD4 p.Gln311Ter (c.931C>T) (from parent cell line).

Homozygous for TP53 p.Arg273His (c.818G>A) (from parent cell line).

STR信息

Amelogenin        X

CSF1PO        11,12

D2S1338        19,23

D3S1358        15,17

D5S818        11,12

D7S820        10

D8S1179        10 (AddexBio; CLS; PubMed=19372543; PubMed=25877200)

10,16 (CCRID; PubMed=11416159)

D13S317        11 (AddexBio; CLS)

11,12 (ATCC; CCRID; Cosmic-CLP; DSMZ; ECACC; KCLB; PubMed=19372543; PubMed=25877200; PubMed=25926053)

D16S539        11,12

D18S51        13

D19S433        14

D21S11        29,30

FGA        20,22

Penta D        11,13

Penta E        14,16

TH01        6,9

TPOX        8,9

vWA        17,19

參考文獻

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Next-generation characterization of the Cancer Cell Line Encyclopedia.

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Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)