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供貨周期	現(xiàn)貨	規(guī)格	T25培養(yǎng)瓶x1 1.5ml凍存管x2
貨號	BFN60810339	應(yīng)用領(lǐng)域	醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)
主要用途	僅供科研

細(xì)胞名稱	人黑色素瘤細(xì)胞MeWo
貨物編碼	BFN60810339
產(chǎn)品規(guī)格	T25培養(yǎng)瓶x1	1.5ml凍存管x2
細(xì)胞數(shù)量	1x10^6	1x10^6
保存溫度	37℃	-198℃
運(yùn)輸方式	常溫保溫運(yùn)輸	干冰運(yùn)輸
安全等級	1
用途限制	僅供科研 3類

培養(yǎng)體系	90%DMEM+10%FBS+1%三抗
培養(yǎng)溫度	37℃	二氧化碳濃度	5%
簡介	人黑色素瘤細(xì)胞MeWo取自78歲男性，該細(xì)胞源于ECACC
注釋	Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: COSMIC cell lines project. Part of: ERK genetic alteration cell panel (ATCC TCP-1033). From: Memorial Sloan Kettering Cancer Center; New York; USA. Doubling time: ~32 hours (PBCF). Microsatellite instability: Stable (MSS) (Sanger). Omics: Array-based CGH. Omics: Deep exome analysis. Omics: Deep quantitative proteome analysis. Omics: Deep RNAseq analysis. Omics: DNA methylation analysis. Omics: HLA class I peptidome analysis by proteomics. Omics: SNP array analysis. Omics: Transcriptome analysis.
基因突變	Homozygous for CDKN2A p.Arg80Ter (c.237_238CC>TT) (c.237_238delinsTT) (p.Pro94Leu; c.280_281CC>TT) (PubMed=17260012; PubMed=23851445; ATCC). FGFR1 p.Pro252Ser (c.754C>T) (PubMed=23851445). Heterozygous for MAPK3 p.Pro246Ser (c.736C>T) (ATCC). TP53 p.Gln317Ter (c.949C>T) (PubMed=17260012; PubMed=23851445).
HLA信息	Class I HLA-A *A02:01,26:01 HLA-B B14:02,38:01* HLA-C *C08:02,12:03 Class II HLA-DP DPB104:01,04:02* HLA-DQ *DQB103:01:01,05:01 HLA-DR DRB111:01,01:02*
STR信息	Amelogenin X,Y CSF1PO 12 (ATCC; CLS; Cosmic-CLP; JCRB; PubMed=25877200) 10,12 (ESTDAB) D3S1358 17 D5S818 12,13 D7S820 10,12 D8S1179 13,15 D13S317 8 (Cosmic-CLP; ESTDAB) 8,9 (ATCC; CLS; JCRB; PubMed=25877200) D16S539 10,12 D18S51 14,17 D21S11 30,32.2 FGA 22 Penta D 10 Penta E 5 (CLS) 5,15 (PubMed=25877200) TH01 7,9 TPOX 8,10 (ATCC; CLS; Cosmic-CLP; JCRB; PubMed=25877200) 9,11 (ESTDAB) vWA 15
參考文獻(xiàn)	PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747 Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A. An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines. Cancer Res. 79:1263-1273(2019) PubMed=31068700; DOI=10.1038/s41586-019-1186-3 Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R. Next-generation characterization of the Cancer Cell Line Encyclopedia. Nature 569:503-508(2019) PubMed=31978347; DOI=10.1016/j.cell.2019.12.023 Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P. Quantitative proteomics of the Cancer Cell Line Encyclopedia. Cell 180:387-402.e16(2020)

青旗（上海）生物技術(shù)發(fā)展有限公司，總部位于上海浦東新區(qū)，依托本地高校資源，逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺，在標(biāo)準(zhǔn)化細(xì)胞庫建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測等科研產(chǎn)品與服務(wù)。我們秉承對用戶負(fù)責(zé)的態(tài)度，以對科研的高度嚴(yán)謹(jǐn)，以嚴(yán)格的質(zhì)量控制，為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)！