化工儀器網(wǎng)
技術文章
CCK8 檢測耐藥細胞株耐藥株 PC9R 吉非替尼藥物的耐藥作用
閱讀：460 發(fā)布時間：2022-10-27
四、 IC50 結(jié)果計算
1、儀器導出原始數(shù)據(jù)
吸光度檢測 | 通道 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
檢測波長 | A | ||||||||||||
450 nm | B | 1.001 | 0.881 | 0.915 | 0.686 | 1.951 | 1.422 | 1.315 | 1.314 | 1.209 | 2.044 | ||
C | 1.226 | 1.254 | 1.166 | 0.581 | 2.273 | 1.503 | 1.478 | 1.508 | 1.112 | 1.973 | |||
檢測日期 | D | 1.57 | 1.544 | 1.543 | 0.522 | 1.929 | 1.661 | 1.803 | 1.743 | 1.143 | 2.145 | ||
2019/8/1 | E | 1.691 | 1.787 | 1.717 | 0.487 | 0.239 | 1.943 | 1.858 | 1.796 | 0.93 | 0.232 | ||
F | 1.811 | 1.769 | 1.838 | 0.399 | 0.234 | 1.941 | 1.859 | 1.896 | 0.857 | 0.244 | |||
檢測時間 | G | 2.094 | 1.838 | 1.905 | 0.525 | 0.25 | 2.077 | 1.903 | 1.963 | 0.851 | 0.232 | ||
16:07:31 | H |
加樣備注：
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | PCR | PC9R | ||||||||||
B | 12.5uM | 12.5uM | 12.5uM | 25uM | 0uM | 12.5uM | 12.5uM | 12.5uM | 25uM | 0uM | ||
C | 6.25uM | 6.25uM | 6.25uM | 25uM | 0uM | 6.25uM | 6.25uM | 6.25uM | 25uM | 0uM | ||
D | 3.125uM | 3.125uM | 3.125uM | 25uM | 0uM | 3.125uM | 3.125uM | 3.125uM | 25uM | 0uM | ||
E | 1.5625uM | 1.5625uM | 1.5625uM | 50uM | 空白 | 1.5625uM | 1.5625uM | 1.5625uM | 50uM | 空白 | ||
F | 0.78125uM | 0.78125uM | 0.78125uM | 50uM | 空白 | 0.78125uM | 0.78125uM | 0.78125uM | 50uM | 空白 | ||
G | 0.390625uM | 0.390625uM | 0.390625uM | 50uM | 空白 | 0.390625uM | 0.390625uM | 0.390625uM | 50uM | 空白 | ||
H |
2、計算結(jié)果
親本株 | 濃度(uM) | 50 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 |
抑制百分比 (%) | 87.32965 | 80.36832 | 61.80479 | 46.16943 | 27.55064 | 17.64273 | 13.53591 | 5.819521 | |
耐藥株 | 濃度(uM) | 50 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 |
抑制百分比 (%) | 64.61313 | 49.46828 | 38.70554 | 30.67473 | 17.51008 | 10.35937 | 8.544188 | 4.015402 |