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產品編號	4527R
英文名稱	CSFV Envelope glycoprotein E2
中文名稱	豬瘟病毒包膜糖蛋白E2抗體
別    名	polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus]; POLG_CSFVA.
研究領域	細菌及病毒
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	CSFV
產品應用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	42kDa
細胞定位	細胞膜
性    狀	Liquid
濃    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from CSFV Envelope glycoprotein E2:951-1050/3898
亞    型	IgG
純化方法	affinity purified by Protein A
儲 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產品介紹	Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins. Envelope glycoprotein E2 exists in the form of homodimer; this protein is cleaved from the polyprotein by a cellular signal peptidase. Function: E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane. P7 forms a leader sequence to properly orient NS2 in the membrane. Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity. RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome. Subunit: The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers. Subcellular Location: E(rns) glycoprotein: Host membrane; Peripheral membrane protein. Note: The C-terminus membrane anchor of Erns represents an amphipathic helix embedded in plane into the membrane Similarity: Belongs to the pestivirus polyprotein family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 peptidase C53 domain. Contains 1 peptidase C74 domain. Contains 1 peptidase S31 domain. Contains 1 RdRp catalytic domain. SWISS: P21530 Gene ID: 920146 Database links: Entrez Gene: 920146 CSFVA SwissProt: P19712 CSFVA Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.