PEGylated protein ELISA kit：

Validated, Complete Assay for the Sensitive Detection of a Diverse Set of PEGs in Biological Samples

•Validated for a wide range of MW linear and branched PEGs, both in free form and when conjugated to proteins

•Highly sensitive, measure as low as <1ng/mL of PEGylated proteins

•Quantify PEGylated target molecules in complex matrices to monitor drug levels or its accumulation in tissue

•High-throughput format, analyze up to 35 samples in duplicate results in just 2 hours

The PEGylated Protein ELISA kit is a competitive assay specific to the backbone of poly(ethylene glycol) (PEG) and has been validated for use with a wide variety of PEG molecules including linear and branched forms as well as free and conjugated forms. As expected, the assay is more sensitive to higher molecular weight PEG molecules due to the repeating characteristics of the backbone. The assay uses a monoclonal antibody to PEG pre-coated on a microtiter plate. The antibody binds in a competitive manner the PEG or PEGylated protein in the sample or the PEG covalently linked to biotin. After incubation at room temperature the excess reagents are washed away and a streptavidin HRP conjugate is added to bind to the biotinylated PEG bound to the antibody. After a second wash to remove excess HRP substrate is added. An HRP-catalyzed reaction gener-ates a blue color in the solution. The reaction is stopped with stop solution and the resulting yellow color is read at 450nm. The amount of signal is inversely proportional to the concentration of PEG.

Product Details

Alternative Name: Polyethylene glycol

Sensitivity:  <1 ng/ml (range 1.75-225 ng/ml)

Assay Time:  2 hours

Applications:  ELISA, Colorimetric detection

Application Notes: For the measurement of PEGylation and quantitative determination of PEGylated molecules in plasma, serum, tissue and other biological samples.

Wavelength:  450 nm

Species reactivity: Species independent

Shipping: Blue Ice Not Frozen

Long Term Storage:  +4°C

Contents: Microtiter Plate, PEG-BSA Assay Control, Biotinylated PEG, Assay Buffer 39, Conjugate, Wash Buffer 3, TMB Substrate, Stop Solution 2, Plate Sealer

艾美捷 聚乙二醇化蛋白質(zhì) ELISA試劑盒是一種針對(duì)聚乙二醇 (PEG) 骨架的競(jìng)爭(zhēng)性檢測(cè)試劑盒，已被驗(yàn)證可用于多種 PEG 分子，包括直鏈和支鏈形式以及游離和共軛形式。正如預(yù)期的那樣，由于骨架的重復(fù)特征，該測(cè)定對(duì)更高分子量的 PEG 分子更敏感。該測(cè)定使用預(yù)涂在微量滴定板上的針對(duì) PEG 的單克隆抗體。抗體以競(jìng)爭(zhēng)方式結(jié)合樣品中的 PEG 或 PEG 化蛋白或與生物素共價(jià)連接的 PEG。在室溫下孵育后，洗去多余的試劑并加入鏈霉親和素 HRP 偶聯(lián)物以結(jié)合與抗體結(jié)合的生物素化 PEG。在第二次洗滌以去除多余的 HRP 底物后，添加。HRP 催化的反應(yīng)在溶液中產(chǎn)生藍(lán)色。用終止溶液終止反應(yīng)并在 450nm 處讀取所得黃色。信號(hào)量與 PEG 濃度成反比。

艾美捷 PEG化蛋白ELISA試劑盒特點(diǎn)：

1.經(jīng)驗(yàn)證適用于各種 MW 線性和支鏈 PEG，無論是游離形式還是與蛋白質(zhì)結(jié)合時(shí)

2.高度靈敏，可測(cè)量低至 <1ng/mL 的聚乙二醇化蛋白質(zhì)

3.量化復(fù)雜基質(zhì)中的 PEG 化靶分子，以監(jiān)測(cè)藥物水平或其在組織中的積累

4.高通量格式，只需 2 小時(shí)即可分析多達(dá) 35 個(gè)樣品，重復(fù)結(jié)果

艾美捷PEG化蛋白ELISA試劑盒化學(xué)性質(zhì)：

備用名稱：聚乙二醇

靈敏度：<1 ng/ml（范圍 1.75-225 ng/ml）

檢測(cè)時(shí)間：2小時(shí)

應(yīng)用：ELISA，比色檢測(cè)

應(yīng)用說明：用于測(cè)量血漿、血清、組織和其他生物樣品中的聚乙二醇化分子和定量測(cè)定聚乙二醇化分子。

波長：450 納米

物種反應(yīng)性：物種獨(dú)立

船運(yùn)：藍(lán)冰未冷凍

長期儲(chǔ)存：+4°C

內(nèi)容：微量滴定板、PEG-BSA 檢測(cè)對(duì)照、生物素化 PEG、檢測(cè)緩沖液 39、偶聯(lián)物、洗滌緩沖液 3、TMB 底物、終止液 2、封板劑

RUO - 僅供研究使用