Recombinant Left/Right Determination Factor 2 (LEFTY2)  

左右決定因子2(LEFTY2)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues:Tyr245~Asp356

Tags:N-terminal His-Tag

Tissue Specificity:Uterus.

Subcellular Location: Secreted

Purity: > 95%

Traits:Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration:200µg/mL

Applications:SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:6.0

Predicted Molecular Mass:13.9kDa

Accurate Molecular Mass:17kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs fromthe predictedareasfollows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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