| CBP71057 | |
| I. Background | |
CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1α, MIP-1? and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium-inducing strains (macrophage-tropic) of HIV-1 virus. | |
| II. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Gene Synonyms: | C-C chemokine receptor type 5, CCR5 |
Expressed Gene: | GenBank accession number NM_000579; no expressed tags |
Host Cell: | CHO-K1 |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F-12+10%FBS+3μg/ml puromycin+100μg/ml Hygromycin B |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for CCR5 receptor |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Concentration-dependent stimulation of intracellular calcium mobilization by MIP-1α in CCR5/Gα15/CHO and Gα15/CHO cells
Figure 1. MIP-1α-induced concentration-dependent stimulation of intracellular calcium mobilization in CCR5/Gα15/CHO and Gα15/CHO cells. The cells were loaded with Calcium-4 prior to stimulation with an CCR5 receptor agonist, MIP-1α. The intracellular calcium change was measured by FlexStation. | |
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