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產品名稱

可溶性I型膠原

應用

用于傳統的二維（2D）系統以及三維（3D）凝膠中的支架，用于模擬成纖維細胞和軟骨細胞中的細胞生長。

規格

4 mg/ml x 12.5 ml (sterile filtered)

形態

溶于0.01M HCL

來源

豬

內毒素

＜1 EU/ml

純度

＞95% I 膠原

保存

4℃

穩定性

6月

操作流程

包被（注：具體的包被的條件需要根據培養系統優化）

1.用0.02mol/L的鹽酸稀釋4mg/ml的膠原到50-100ug/ml。

2.溫和混勻稀釋后的溶液。

3.取適量的稀釋后的膠原加入孔內或板內。

注：保證整個表面都是包被的。

4. 37℃或室溫孵育1-2小時

5. 棄掉所有溶液。

6.用培養基或者PBS漂洗包被表面。

7.包被好的孔或板2-8℃保存或者空氣中晾干（如果保持無菌）

三維凝膠制備流程：

1. 用PBS等體積稀釋膠原溶液，終濃度2mg/ml。
2. 加入適量的稀釋后的膠原到孔內或板內。
3. 37℃孵育30~60分鐘。
4. 凝膠可以2-8℃保存或者空氣中晾干（如果保持無菌）。

參考文獻

1. M. Shoulders, R. Raines, Collagen structure and stability. Annu Rev Biochem 78, 929-58 (2009).

2. W. Friess, Collagen--biomaterial for drug delivery. Eur J Pharm Biopharm 45, 113-36 (1998).

3. Taubenberger, M. Woodruff, H. Bai, D. Muller, D. Hutmacher, The effect of unlocking RGD-motifs in collagen I on pre-osteoblast adhesion and differentiation. Biomaterials 31, 2827-35 (2010).

4. D. Gullberg, K. Gehlsen, D. Turner, K. Ahlén, L. Zijenah, et al., Analysis of alpha 1 beta 1, alpha 2 beta 1 and alpha 3 beta 1 integrins in cell--collagen interactions: identification of conformation dependent alpha 1 beta 1 binding sites in collagen type I. EMBO J 11, 3865-73 (1992).

5. J. Bard, E. Hay, The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro. J Cell Biol 67, 400-18 (1975).

6. H. Kleinman, R. Klebe, G. Martin, Role of collagenous matrices in the adhesion and growth of cells. The Journal of cell biology 88, 473-485 (1981).

7. J. Tomasek, E. Hay, Analysis of the role of microfilaments and microtubules in acquisition of bipolarity and elongation of fibroblasts in hydrated collagen gels. J Cell Biol 99, 536-49 (1984).

8. D. Karamichos, N. Lakshman. W.Petroll, Regulation of corneal fibroblast morphology and collagen reorganization by extracellular matrix mechanical properties. Investigative ophthalmology & visual science 48, 5030-5037 (2007).

9. S. Oliveira, R. Ringshia, R. Legeros, E. Clark, M. Yost, et al., An improved collagen scaffold for skeletal regeneration. J Biomed Mater Res A 94, 371-9 (2010).