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產品名稱：Invitrogen 18080-300 SuperScriptTM III CellsDirect cDNA Synthesis Kit
貨 號：18080-300
規 格：100 rxns
分 類：cDNA 合成試劑盒
品 牌：Invitrogen
國產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態：現 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業供應Invitrogen 18080-300 SuperScriptTM III CellsDirect cDNA Synthesis Kit，常備現貨，*。主營Life Invitrogen產品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試劑、培養基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
cDNA Synthesis Kit描述：
The SuperScript? III CellsDirect cDNA Synthesis Kit is optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR. For real-time quantitative RT-PCR, see the note below. The kit has the following advantages:
? Compatible with a wide range of mammalian cell types grown under different treatment conditions
? Single-tube format minimizes reagent loss, sample loss, and handling time
? Total lysate volume is used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
? SuperScript? III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
? Generates high-quality cDNA for use in a variety of applications, including cloning and PCR
? Simple protocol takes less than 2 hours
Note: For real-time quantitative RT-PCR (qRT-PCR) from cell lysate, we recommend the SuperScript? III Platinum? CellsDirect Two-Step qRT-PCR Kit (Cat. Nos. 11737-030 and 11737-038) or the SuperScript? III Platinum? CellsDirect Two-Step qRT-PCR Kit with SYBR? Green (Cat. Nos. 11738-060 and 11738-068). These kits include reagents and protocols that have been specifically optimized for real-time qRT-PCR.
cDNA Synthesis Kit同類產品主要有:
| 貨號 | 名稱 | 規格 |
| 18064-014 | SuperScript? II Reverse Transcriptase | 10,000U |
| 18080-044 | SuperScript? III Reverse Transcriptase | 10,000U |
| 18418-012 | Oligo(dT)12-18 Primer | 25ug |
| 18418-020 | Oligo(dT)20 Primer | 50ul(50uM) |
| 10777-019 | RNaseOUTTM Ribonuclease Inhibitor | 5,000U |
| 18080-051 | SuperScript? III First-Strand | 50 rxn |
| Synthesis System for RT-PCR | ||
| 11668-027 | Lipofectamine TM 2000 Reagent | 0.75ml |
| 11668-019 | 1.5ml | |
| 13778-150 | Lipofectamine RNAiMax | 1.5ml |
| 13778-075 | Lipofectamine RNAiMax | 0.75ml |
| 15596-026 | TRlzol ?Reagent | 100ml |
| 15596-018 | TRlzol ?Reagent | 200ml |
| 10296028 | TRIZOL LS Reagent 200 ml（長方形） | 200ml |
| 10296010 | TRIZOL LS Reagent 100ml（長方形） | 100ml |
| 11789020 | BP CLONASE II ENZYME MIX | 20 REACTIONS |
| 11791020 | LR CLONASE II ENZYME MIX | 20 REACTIONS |
| 12328040 | AMV | 100 REACTIONS |
| (CLON AMV FIRST STR SYNTH KIT) | ||
| 28025-013 | M-MLV Reverse Transcriptase | 40,000U |
| 17504-044 | B-27（黃顏色） | 10ml |
| 12587-010 | B-27（黃顏色）不含維生素A | 10ml |
| 15090-046 | 2.5% Trypsin (10X), no Phenol Red | 100ml |
| 15050-065 | 0.25% Trypsin(1X),Phenol Red | 100ml |
| 15050-057 | 500ml | |
| 15140-122 | Pen Strep | 100ml |
| 15140-163 | 20X100ml | |
| 25200-056 | 0.25% Trypsin-EDTA(1X,Phenol Red) | 100ml |
| 25200-114 | 20X100ml | |
| 25300-054 | 0.05% Trypsin(1X),Phenol Red: | 100ml |
| 25300-120 | 20X100ml | |
| 15400-054 | 0.5% Trypsin(10X),: | 100ml |
| 25030-081 | L-Glutamine 200mM | 100ml |
| 25030-164 | 20X100ml | |
| 12328019 | CLONED AMV RT, 750U | TRANSCRIPTASE |
| 12328032 | CLONED AMV FIRST STRA SYN KIT | 25 REACTIONS |
| 10966-018 | Platinum? Taq DNA聚合酶 | 100 rxns |
| 10966-026 | 250 rxns | |
| 10966-034 | 500 rxns | |
| K210007 | PURELINK PLASMID MAXI 25 | 25 REACTIONS |
| 15224041 | T4 DNA LIGASE HI CONC. | 250 U |
| C737303 | ONE SHOT STBL3 COMP E COLI | ONE SHOT STBL3 20 RXN |
| 12033015 | ELECTROMAX DH10B T1 CELLS | 0.5 ML (5 X 0.1 ML) |
| A10460 | ONE SHOT CCDB SURVIVAL 2-T1R | 10 REACTIONS |
| LC5925 | SEEBLUE PLUS2 | 500UL |
| 11708039 | PLATINUM PFX DNA POLYMERASE | 500 REACTIONS #95514- #52806 |
| 12344024 | ACCUPRIME PFX DNA POLYMERASE | 200 REACTIONS |
| 12346086 | ACCUPRIME TAQ HIFI | 200 RXNS |
| 11495017 | PCRX ENHANCER SYSTEM | 250 RXN 52395 |
| 450008 | TOPO XL PCR CLONING KIT | 10 RXN |
| MECOV50 | METHYLCODE BISULFITE CONV KIT | 50 REACTIONS |
| 12935300 | STEALTH RNAI NEG CTL MED GC | 250 μl |
| 2013 | FLUORESCENT OLIGOMER, 20UM | 250 UNITS |
| K159303 | KIT,FAST TRACK | 18 RXN |
| 11917020 | SS DBL STRND CDNA SYN KIT | 50 RXNS |
| LC5677 | MARK12 W RANGE MW STD,1ML | 1 ML |
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