【蛋白交聯劑的分類】：

1：同源交聯劑：

具有兩個相同活化反應基團。應用：一步反應，分子內交聯，多聚反應，穩定四級結構

2：異源交聯劑：

具有兩個不同活化反應基團。應用：兩步反應交聯，可減少聚集和自身交聯。反應原則：先反應不穩定活性基團（與*種蛋白質），然后去除掉未反應的交聯劑，在加入種待交聯蛋白質，短臂用于分子間交聯，長臂用于分子內交聯反應：SH……NH2， NH2…… COOH

3：光反應交聯劑：

反應原則：先交聯不穩定的熱反應活性基團（與*種蛋白質），然后UV交聯，將*種蛋白質交聯到種蛋白質的N-H，C-H上。特點：交聯非特異性，產率低。

【交聯劑的使用方法】：

圖示為同源交聯劑的使用方法：先將待交聯的蛋白質A與交聯劑在一定條件下反應，得到的蛋白質A-交聯劑中間體，然后移除多余的交聯劑，再加入欲交聯的蛋白質B。產物中可能會形成多聚體，這一副產物通過調節反應條件來控制。分成兩部反應的目的在于減少分子內交聯以及形成蛋白質多聚物。另外，選擇異源交聯劑也可以減少多聚物的產生。

【交聯劑的應用】：

膜結構研究

蛋白質結構研究

蛋白質間相互作用研究

生物研究（靶向）

載體蛋白與半抗原的連接

蛋白質或其他分子的固相化

抗體的標記、標記轉移

蛋白質與核酸的連接

蛋白質修飾等 

【選擇交聯劑應考慮的因素】：

反應特異性

反應指向性

間臂長度性

水溶性

透膜性

一種或兩種反應基團

熱反應還是光電反應

可否切斷

可否碘標

【可與交聯劑反應的基團】：

游離氨基（賴氨酸ε或N末端的氨基）

巰基（半胱氨酸）

羧基（天冬氨酸，谷氨酸，末端羧基）

羥基（或蘇氨酸）

酮基，醛基，糖鏈

苯酚衍生物（苯丙氨酸，色氨酸，酪氨酸）

放射性基團: R-N3 

交聯劑反應基團和反應指向性一覽

西安瑞禧生物科技有限公司

Xi'an ruixi  Biological Technology Co.,Ltd

：     ：  號：harrycr1982

產品列表：

Biotin-hydrazide               66640-86-6

Biotin-LC-NHS Ester           72040-63-2

Biotin-PEG3-CH2CH2NH2   359860-27-8

Biotin-LC                            72040-64-3

Biotin-ONP                         33755-53-2

Biotin-PEG4-CH2CH2N3     875770-34-6

Biotin-LC-LC                      89889-51-0

Biotin-LC-Hydrazide            109276-34-8

Biotin-NHS Ester                 35013-72-0

NHS-SS-(+)-Biotin                     142439-92-7

LC-(+)-Biotin hydrazide    109276-34-8

GMBS                  80307-12-6

EMCA                                55750-53-3

SMPB                                 79886-55-8

BMPS                                 55750-62-4

sulfo-LC-(+)-Biotin        127062-22-0

Biotin-PFP-ester          120550-35-8

BMME                                15209-14-0

SMCC hydrazide                  181148-00-5

Sulfo-SMCC              92921-24-9

Sulfo-NHS               106627-54-7

SBAP                                 57159-62-3

HSAB                                 53053-08-0

DSS                                    68528-80-3

EMCS                                 55750-63-5

SIA                                    39028-27-8

LC-SMCC                           125559-00-4

BS3                                    82436-77-9

Sulfo-SANPAH         CAS Number: 102568-43-4

MBS                  CAS Number: 58626-38-3

SPDP                   CAS Number: 68181-17-9

LC-SPDP

Sulfo-LC-SPDP

Sulfo-NHS-Biotin            CAS Number: 119616-38-5

Sulfo-NHS-LC-Biotin  CAS Number: 127062-22-0

NHS-LC-Biotin       CAS Number: 72040-63-2

Sulfo-NHS-SS-Biotin         CAS Number: 325143-98-4

NHS-SS-Biotin       CAS Number: 142439-92-7

Biotin Hydrazide      CAS Number: 66640-86-6

Biotin-HPDP          CAS Number: 129179-83-5

Iodoacetyl-LC-Biotin  CAS Number: 93285-75-7

Biotin–cadaverine

PFP-Biotin

Sulfo-NHS-SS-Biotin

Sulfo-NHS-LC-LC-Biotin     127062-22-0

Sulfo-NHS-LC-Biotin           127062-22-0

NHS-LC-LC-Biotin              89889-52-1

DSS Crosslinker                 CAS Number: 68528-80-3

DSG Crosslinker                  CAS Number: 79642-50-5

DSP Crosslinker                  CAS Number: 57757-57-0

DTSSP Crosslinker                CAS Number: 81069-02-5

Sulfo-EGS Crosslinker             CAS Number: 167410-92-6

EGS Crosslinker                  CAS Number: 70539-42-3

DST Crosslinker                  CAS Number: 62069-75-4

生物交聯試劑產品目錄：

R-S-0001   3-Maleimidopropionic acid                   7423-55-4

R-S-0002   (DTSP);Dithio-bis-succinimidyl propionate      57757-57-0

R-S-0003   D-甲酯                             60562-11-0

R-S-0004   (SFB);Succinimidyl-p-formyl-benzoate         60444-78-2

R-S-0005   Disuccinimidyl sebacate                     23024-29-5

R-S-0006   p-Azidobenzoic acid                        6427-66-3

R-S-0007   FMOC-D-LYS(BIOTIN)-OH                 110990-09-5

R-S-0008   NHS-SS-(+)-Biotin                         142439-92-7

R-S-0009   LC-(+)-Biotin hydrazide                     109276-34-8

R-S-0010   S-Acetylmercaptosuccinic anhydride           142439-92-7

R-S-0011   D-甘氨酸                           16390-90-9

R-S-0012   N-Biotinyl-1,6-hexanediaMine                65953-56-2

R-S-0013   biotinylamidoethylacetamide                  111790-37-5

R-S-0014   (+)-Biotin-NHS;BIOTIN-NHS                 35013-72-0

R-S-0015   Biotin hydrazide                            66640-86-6

R-S-0016   (GMBS);4-Maleimidobutyric acid NHS         80307-12-6

R-S-0017   (N-Maleoyl-GABA);4-Maleimidobutyric acid     57078-98-5

R-S-0018   (EMCA);6-Maleimidocaproic acid              55750-53-3

R-S-0019   (+)-Biotin;USP                             58-85-5

R-S-0020   sulfo-LC-(+)-Biotin                          127062-22-0

R-S-0021   Biotin-丁氨酸-NHS                         258289-54-2

R-S-0022   (+)-Biotin-X-X-NHS                        89889-52-1

R-S-0023   (MBS-1);m-Maleimidobenzoyl N-hydroxysuccinimide;   58626-38-3

R-S-0024   (SMPB);Succinimidyl 4-(p-maleimidophenyl)butyrate    79886-55-8                    

R-S-0025   a-Maleimidoacetic acid-NHS(AMAS)                 55750-61-3

R-S-0026   2-Maleimidoethyl amine                        146474-00-2

R-S-0027   (BMPS);3-Maleimidopropionic acid NHS          55750-62-4

R-S-0028   (+)-Biotin-PFP-ester                           120550-35-8

R-S-0029   4-(N-Maleimido)benzophenone                  92944-71-3

R-S-0030   5-DTAF;5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein hydrochloride 51306-35-5

R-S-0031   (SANPAH);N-Succinimidyl-6-(4'-azido2'-nitrophenyl-amino)hexanoate 64309-05-3

R-S-0032   1,4-bis-Maleimidobutane                        28537-70-4

R-S-0033   1,6-bis-Maleimidohexane                        4856-87-5

R-S-0034   (BMME);bis-Maleimidomethylether               15209-14-0

R-S-0035   11-Maleimidoundecanoic acid                    57079-01-3

R-S-0036   mono-(BOC)-ethylene diamine                   57260-73-8

R-S-0037   (DCC);N,N'-Dicyclohexylcarbodiimide            538-75-0

R-S-0038   (PFPC)Pentafluorophenyl carbonate               59483-84-0

R-S-0039   Methyl methanethiolsulfonate                    2949-92-0

R-S-0040   Iodoacetic anhydride                           54907-61-8

R-S-0041   SMCC hydrazide                              181148-00-5

R-S-0042   5(6)-carboxyfluorescein diacetate                 124387-19-5

R-S-0043   sulfo-SMCC                                  92921-24-9

R-S-0044   sulfo-NHS                                    106627-54-7

R-S-0045   (+)-Camptothecin                               7689-03-4

R-S-0046   SBAP;Succinimidyl 3-(bromoacetamido)propionate   57159-62-3

R-S-0047   (HSAB);N-Hydroxysuccinimidyl 4-azidobenzoate    53053-08-0

R-S-0048   3-Nitro-4-fluorophenylazide                      28166-06-5

R-S-0049   p-Azidophenacyl bromide                       57018-46-9

R-S-0050   ANB-NOS;N-(5-Azido-2-nitrobenzoyloxy)succinimide 60117-35-3

R-S-0051   (DSS);Disuccinimidyl suberate                   68528-80-3

R-S-0052   Di-tert-butyl dicarbonate                        24424-99-5

R-S-0053   NHS;N-Hydroxysuccinimide                     6066-82-6

R-S-0054   HEPES;(N-2-Hydroxyethylpiperazine N'-2-ethanesulfonic acid) 7365-45-9

R-S-0055   DMT-Cl;4,4'-Dimethoxytrityl chloride             40615-36-9

R-S-0056   PIPES;Piperazine N,N'-bis-2-ethanesulfonic acid     5625-37-6

R-S-0057   TRIS:Molecular Biology Grade tris-(Hydroxymethyl)aminomethane 77-86-1

R-S-0058   Colchicine                                    64-86-8

R-S-0059   FORSKOLIN                                 66575-29-9

R-S-0060   4-BENZOYLBENZOIC ACID SUCCINIMIDYL ESTER   91990-88-4

R-S-0061   4-Hydroxyphenyl glyoxal;(HPG)                  197447-05-5

R-S-0062   (EMCS);6-Maleimidocaproic acid NHS            55750-63-5

R-S-0063   (SIA);Succinimidyl iodoacetate                   39028-27-8

R-S-0064   (FMOC-OSu); N-(9-Fluorenylmethoxycarbonyloxy)succinimide 82911-69-1

R-S-0065   (SBA); Succinimidyl bromoacetate                42014-51-7

R-S-0066   TES;N-tris-(hydroxymethyl) methyl-2-aminoethane sulfonic acid 7365-44-8

R-S-0067   (-)-Podophyllotoxin                            518-28-5

R-S-0068   MES;2-(N-morpholino)ethanesulfonic acid         4432-31-9

R-S-0069   10-Deacetyl baccatin III                        32981-86-5

R-S-0070   LC-SMCC                                   125559-00-4

R-S-0071   Succinimidyl-5(6)-carboxyfluorescein             117548-22-8

R-S-0072   BS3;bis-sulfo-succinimidyl suberate               82436-77-9