Renca 小鼠腎癌細胞 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規范,提供 *培養條件,
ATCC® Number: CRL-2947™ Price: $379.00
Designations: Renca
Depositors: R.Wiltrout
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus （mouse）
Morphology: epithelial-like
Source: Organ: kidney
Disease: renal adenocarcinoma
Cell Type: epithelial
Strain: Balb/cCr
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Isolation: Isolation date: 1969
Tumorigenic: YES
Age: 6 weeks
Gender: male
Comments: The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice. The pattern of growth of this tumor accuray mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. [PubMed: 4703766, 4057425]The cells do not express transforming growth factor-beta type II receptor （TbetaR-II）. [PubMed: 10414746] Renca 小鼠腎癌細胞
Propagation: ATCC complete gro wth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
10% fetal bovine serum （final conc.）
non-essential amino acids （NEAA） （0.1mM extra）
additional sodium pyruvate （1mM extra）
additional L-glutamine （2mM extra）
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS） or 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10（4） to 4 X 10（4） viable cells/sq. cm is recommended.
Incubate cultures at 37C. We recommend that you subculture when the culture reaches a cell concentration between 8 X 10（4） and 1.5 X 10（5） cells/sq. cm.
Subc*tion ratio: A subc*tion ratio of 1:4 to 1:10 is recommended.
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5%
Storage temperature liquid nitrogen vapor phase
Doubling Time: approximay 24 hours
Related Products: Recommended medium （without the additional serum described under ATCC Medium）: ATCC 30-2001
Recommended serum: ATCC 30-30-2020
0.25% （w/v） Trypsin - 0.53mM EDTA in Hank's BSS （w/o Ca++, Mg ++）: ATCC 30-2101
Phosphate-buffered saline: ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
L-Glutamine solution, 200mM: ATCC 30-2214
Erythrosin B vital stain solution: ATCC 30-2404
References: 16172681: Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50（4）:1013-25, 1973. PubMed: 4703766
16172682: Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134（6）:1236-41, 1985. PubMed: 4057425
16172683: Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma （renca） cells . Urology. 54（1）:164-70, 1999. PubMed: 10414746
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