CRL-1420 MIA PaCa-2 人胰腺癌細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和*培養條件！
CRL-1420 MIA PaCa-2 人胰腺癌細胞
ATCC® Number: CRL-1420™ Price: $279.00
Designations: MIA PaCa-2
Depositors: A Yunis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, single cells and loosely attached clusters
Organism: Homo sapiens （human）
Morphology: attached epithelial with floating rounded cells
CRL-1420 MIA PaCa-2 人胰腺癌細胞
Source: Organ: pancreas
Disease: carcinoma
Cellular Products: human colony stimulating factor, subclass I （CSF-I）; plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
DNA Profile （STR）: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
Isoenzymes: G6PD, B
Age: 65 years
Gender: male
Ethnicity: Caucasian
Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034]
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034]
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 （F1A3-23） and ATCC HB-8208 （F18 AF1）.
The line is sensitive to asparaginase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line （MIA PaCa-2）. J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
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