immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
Permits/Forms:
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Tumorigenic:
Yes
Comments:
Animals were immunized with human mesenchymal stem cells. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 20%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020. Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days
recommended serum:ATCC 30-2020 Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X
References:
22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996 49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137
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