Organ: spleen Strain: BALB/c Cell Type: hybridoma: B lymphocyte;
Permits/Forms:
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Applications:
fusion partner for production of somatic cell hybrids
Antigen Expression:
H-2d
Comments:
The line was formed by fusing BALB/c spleen cells （from mouse immunized with sheep RBCs） with the P3X63Ag8 myeloma. [1077] The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. [1077] Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. [906] Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 10 （4） and 5 X 10 （5） viable cells/ml. Some cells can attach and be transferred by shaking them loose. Medium Renewal: Add fresh medium every 2 to 4 days （depending on cell density）
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC CRL-1581.1
References:
906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293 1077: Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 （spi-1） promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
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