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[供應(yīng)]BNL CL.2 小鼠胚胎肝細(xì)胞

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更新時(shí)間:2025-01-22 21:00:06

有效期:2025年1月22日 -- 2025年7月22日

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BNL CL.2 小鼠胚胎肝細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!
BNL CL.2 小鼠胚胎肝細(xì)胞 的詳細(xì)介紹

BNL CL.2 小鼠胚胎肝細(xì)胞

ATCC® Number:  TIB-73™       
Designations:  BNL CL.2 
Depositors:   P Patek, J Collins, M Cohn 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Mus musculus (mouse) 
Morphology:
 
Source: Organ: liver
Strain: BALB/c
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Tumorigenic: No 
Age:  embryo 
Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus (mousepox).
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
 
Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: derivative:ATCC TIB-74
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 
 
 


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