美國Oct4-GFP報告基因小鼠胚胎干細胞
美國PrimCells干細胞：PrimCells提供多種報告基因敲進的小鼠胚胎干細胞，該類細胞皆在原始基因座上敲入熒光報告基因，從而使熒光蛋白的表達能真實反映原基因座附近的表達調控狀況。該類細胞作為研究胚胎干細胞自更新和定向分化的有力工具，受到多家*研究所研究人員的青睞。
Oct4 is a transcriptional factor from POU protein family. It is one of the most important markers for undifferentiated embryonic stem cells and is required to maintain the self-renwal of mESCs1. Oct4 is also associated with human germ cell tumors2.
PrimCells is proud to isolate and provide to the research community the culture of Oct4-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the mechanism of self-renwal of mouse embryonic stem cells. Oct4-GFP mESCs should be maintained in the stem cell culture medium with LIF to maintain the pluripotent state (Figure 1).
Note: This cell line is puromycin-resistent.
Figure 1. Oct4-GFP mESCs show GFP signals when maintained in ESC medium (Left). The differentiated cells lose the GFP signals in the absence of LIF (Right).
References
1. Loh, Y.H. et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38, 431-440 (2006).
2. Looijenga, L.H. et al. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63, 2244-2250 (2003).
美國PrimCells品牌介紹
PrimCells是位于美國加州圣迭戈的一家專業致力于細胞產品研發和解決方案提供的業內技術。
公司匯集了*的生物醫藥技術專家，獨立開發了多項原代細胞和干細胞相關的專有技術。 公司以“細胞研究的*選擇”為宗旨，自主研發超過70種創新產品，*多個特種細胞市場的空白。例如其誘導性表達細胞庫和高效3D皮膚培養系統，皆為該類產品目前市場上少數的規模化供應商。此外，公司以高質量的數據與快速穩定的項目完成時間，長期為多家制藥公司提供解決方案，獲得用戶廣泛贊譽。
PrimCells產品線：
1. 報告基因干細胞 2. 原代細胞（含報告基因原代細胞） 3. 誘導性穩定細胞株
4. 人源重組蛋白 5. 創新型培養試劑
PrimCells 解決方案
1.誘導性穩定細胞株開發 2. 原代細胞開發 3. 藥物體外毒理和效能測試 4. 3D-皮膚培養
PrimCells合作伙伴及客戶:
A．生物制藥公司：輝瑞(Pfizer), 諾華(Novartis), 禮來(Eli2 Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和醫藥研發部門
B．科研機構：冷泉港實驗室，哈佛醫學院, 麻省理工（MIT）,， UCSF, NYU, Salk, UIUC, USC等
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