| Preceptrol? Culture | | Organism: | Geobacillus stearothermophilus （Donk） Nazina et al. deposited as Bacillus stearothermophilus Donk | | Depositor: | CP Hegarty | | Biosafety Level: | 1 | | Shipped: | freeze-dried | | Growth Conditions: | ATCC medium3: Nutrient agar or nutrient broth
Temperature: 55.0°C | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Cross References: | Nucleotide （GenBank） : AF184067 Bacillus stearothermophilus MetS （metS） gene, partial cds; transition state regulator （abrB） gene, complete cds; and yabC pseudogene, partial sequence.
Nucleotide （GenBank） : L13418 Bacillus stearothermophilus maltose permease （malA） gene, complete cds.
Nucleotide （GenBank） : M16814 B.stearothermophilus small acid-soluble spore protein （B-type SASP） gene, complete cds.
Nucleotide （GenBank） : M86508 Bacillus stearothermophilus proton glutamate symport protein （gltP） gene, complete cds.
Nucleotide （GenBank） : M13061 B.stearothermophilus SASP-1 gene encoding a small acid-soluble spore protein, complete cds.
Nucleotide （GenBank） : U18943 Bacillus stearothermophilus mannitol transport protein （MtlA）, putative transcriptional regulator （MtlR）, mannitol enzyme IIA （MtlF） and mannitol-1-phosphate dehydrogenase （MtlD） genes, complete cds. | | Comments: | This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev., <1211>, pp. 1976-1981, 1995 where only the taxon is specified.
Stated to grow at 35C [8243]
The ATCC lab was unable to verify growth at 35C. | | Applications: | gas sterilization control [21513]
steam sterilization control [21513] [11021] [21599] [5923]
sterility assurance [92393] [92308]
sterilization control [92808] [92807] | | References: | 5923: Smith NR, et al. Aerobic spore forming bacteria. U.S. Dep. Agric. Monogr. 16: 1-148, 1952.
8243: Arch. Biochem. Biophys. 54: 154-161, 1955.
11021: British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003
21513: European Pharmacopoeia Commission Biological indicators of sterilization. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.2, 1997
21599: U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 2005
92308: Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.
92393: Sterilization of health care products--Biological indicators--Part 3: Biological indicators for moist heat sterilization.. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 11138-3:1995.
92807: Sterilization of health care products --- Biological indicators, Part 3: Biological indicators for moist heat sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-3:2006.
92808: Sterilization of health care products --- Biological indicators, Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-5:2006.
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