| Designations: | NCTC clone 929 [L cell, L-929, derivative of Strain L] |
| Depositors: | WR Earle |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Mus musculus （mouse） |
| Morphology: | fibroblast
 |
| Source: | Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
| Isolation: | Isolation date: March, 1948 |
| Applications: | testing [92346] [92380] [92382] [92389] [92404]
toxicity testing [21469] [21470] [21606]
transfection host （Nucleofection technology from Lonza
Roche FuGENE? Transfection Reagents） |
| Virus Resistance: | poliovirus 1, 2, 3; coxsackievirus B5; polyomavirus |
| Tumorigenic: | Yes |
| Reverse Transcript: | positive |
| Antigen Expression: | H-2k |
| Cytogenetic Analysis: | modal chromosome number = 66; range = 65 to 68. There were approximay 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. |
| Age: | 100 days |
| Gender: | male |
| Comments: | NCTC clone 929 （Connective tissue, mouse） Clone of strain L was derived in March, 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed.
The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse. [25770]
Clone 929 was established （by the capillary technique for single cell isolation） from the 95th subculture generation of the parent strain. [21404]
Tested and found negative for ectromelia virus （mousepox）. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C |
| Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase |
| Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
derivative:ATCC CCL-1.1
derivative:ATCC CCL-1.2
derivative:ATCC CCL-1.3
derivative:ATCC CCL-1.4 |
| References: |
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