| Designations: | MC3T3-E1 Subclone 4 | | Depositors: | RT Franceschi | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Mus musculus （mouse） | | Morphology: | fibroblast
| | Source: | Organ: bone
Strain: C57BL/6
Tissue: calvaria
Cell Type: preosteoblast; | | Cellular Products: | collagen [51540] | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Tumorigenic: | Yes | | Age: | newborn newborn | | Comments: | A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 （ATCC CRL-2593） and the MC3T3 Subclone 14 （ATCC CRL-2594） lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix （ECM） after 10 days [PubMed ID: 10352097].
The MC3T3 Subclone 24 （ATCC CRL-2595） and the MC3T3 Subclone 30 （ATCC CRL-2596） lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14 [PubMed ID: 10352097].
Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein （BSP）, osteocalcin （OCN）, and the parathyroid hormone （PTH）/parathyroid hormone-related protein （PTHrP） receptor.S ubclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor [PubMed ID: 10352097]. |
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