低價供應 CRL-1469 PANC-1人胰腺癌細胞 CRL-1469

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CRL-1469 PANC-1人胰腺癌細胞
ATCC? Number:  CRL-1469?      
Designations:  PANC-1  
Depositors:   M Lieber  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: pancreas 
Tissue: duct 
Disease: epithelioid carcinoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 CRL-1469 PANC-1人胰腺癌細胞
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE? Transfection Reagents) 
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10,12 
D13S317: 11 
D16S539: 11 
D5S818: 11,13 
D7S820: 8,10 
THO1: 7,8 
TPOX: 8,11 
vWA: 15 
Cytogenetic Analysis: Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%. 
Isoenzymes:  G6PD, B 
Age:  56 years  
Gender:  male  
Ethnicity:  Caucasian  
Comments: Growth is inhibited by 1 unit/ml L-asparaginase. 
The cells will grow in soft agar. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks. 
Preservation:  culture medium 95%; DMSO, 5% 
Doubling Time:  52 hrs  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020 
References: 22850: Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870
22859: Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 
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HeLa    人宮頸癌細胞
Hepa1-6    小鼠肝癌細胞
HepG2    人肝癌細胞
hFOB1.19    人永生化成骨細胞
HGC-27    人胃癌細胞
Hs 746T    人胃癌細胞
HT-29    人結(jié)腸癌細胞
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MCF-7    人乳腺癌細胞
MDA-MB-231    人乳腺癌細胞
MGC80-3    人胃癌細胞
MKN-45    人胃癌細胞
NCI-H460    人大細胞肺癌細胞
NIH-3T3    小鼠成纖維細胞
NUGC-3    人胃癌細胞
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BT-474    人乳腺導管癌細胞
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 CRL-1469 PANC-1人胰腺癌細胞
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HN13    口腔鱗狀細胞癌細胞
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MKN-45    人胃癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
Hs 746T    人胃癌細胞
Hepa1-6    小鼠肝癌細胞
HCT-8    人回盲腸癌細胞
BEL7402 人肝癌細胞系
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GES-1    人胃上皮細胞
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293-L.P.    人胚腎細胞

 CRL-1469 PANC-1人胰腺癌細胞