當前位置:上海一基實業有限公司>>技術文章>>潮霉素B/濕霉素乙
英文名稱:Hygromycin B;O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-o-beta-D-talopyranosyl-(1-5)-2-deoxy-N3-methyl-D-streptamine
CAS號:31282-04-9
C20H37N3O13=527.52
級別:Ultra pure grade
含量:≥80%
濃度:50mg/ml
Working concentration: 50~1000µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used
LD50:6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.);63 mg/kg in rat (i.p.)
性狀:液體,50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
用途:生化研究。是一種氨基糖苷類抗生素,通過阻斷多肽的合成,抑制多肽延長,用于原核和真核細胞的篩選。Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
保存:2~8℃
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