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上海起發實驗試劑有限公司
中級會員·16年
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- 楊經理
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世界*實驗材料供應商BPS Bioscience正式授權上海起發為其中國代理，BPS Bioscience 在一直是行業的*,一直為廣大科研客戶提供zui為的產品和服務,上海起發一直秉承為中國科研客戶帶來的產品，的服務， 簽約BPS Bioscience 就是為了給廣大科研客戶帶來更加完善的產品和服務,您的滿意將是我們zui大的收獲
BPS Bioscience 中國代理，BPS Bioscience 上海代理，BPS Bioscience北京代理，BPS Bioscience 廣東代理，BPS Bioscience 江蘇代理 BPS Bioscience 湖北代理, BPS Bioscience 天津, BPS Bioscience 黑龍江代理, BPS Bioscience內蒙古代理, BPS Bioscience 吉林代理, BPS Bioscience 福建代理, BPS Bioscience 江蘇代理, BPS Bioscience 浙江代理, BPS Bioscience 四川代理,
美國BPS Bioscience公司是一家快速發展的企業，總部在California.產品遠銷世界各地.BPS提供產品和服務,主要方向是蛋白激酶,磷酸激酶，HDAC等.
BPSzui大的藥物發現用重組酶的供應商，并提供蛋白表達和基于細胞和生物化學檢測的產品定制服務和化合物篩選服務。BPS在不斷擴大其藥物發現產品。
BPS還提供了自定義的蛋白表達，生物化學和基于細胞的檢測，化合物篩選服務。 BPS是顧客導向。以及為客戶提供服務，我們傾聽您的需要定制我們的服務，以滿足您的具體情況。
通過BPS的重點在技術發展的zui前沿，的質量生命科學產品和服務提供及時，這將有助于我們的客戶加快藥物發現和開發用于治療人類疾病。
EZH2/EED/SUZ12/RbAp48/AEBP2, FLAG-tag, His-tag
Catalog #: 51004
Size: 50 µg
Species: Human
Host Species: Sf9 insect cells
MW: 325 kDa (complex)
Genbank #: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Tag(s): N-terminal FLAG-tag(EED), N-terminal His-tag (other components)
a.a: 2–end (all components)
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 protein complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 protein complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
UniProt Q15910
同義字（詞）：EZH2 Protein Complex, EZH2, PRC2, Enhancer Of Zeste Homolog 2; RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), NURF55, Chromatin Assembly Factor 1 Subunit C, CAF1 p48; Embryonic Ectoderm Development, hEED, EED, WAIT-1; SUZ12, Suppresso
Specific Activity: 0.08 pmol/min/µg
測定條件：50 µl reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 20 µM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format: Aqueous buffer solution
儲存/穩定性：>6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Morin, R., et al. (2010). et al., Nature Genetics. 42, 181 - 185.
2. Varambally, S. et al. (2008). Science 322 (5908), 1695-1699.
3. Rakotobe, D. et al. (2008). Virol. J. 5, 32.
Application Reference(s):
1. Arcand, M. et al., Poster presented as part of the BioTechniques Fall 2011 Epigenetics Meeting. , et al., ""Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases."" Poster presented as part of the BioTechniques Fall 2011 Epigenetics Meeting.
2. Cohen, A. et al., Weaver Syndrome-Associated EZH2 Protein Variants Show Impaired Histone Methyltransferase Function In Vitro (2016)
3. Yap, D.B, et al., Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethlyation. (2010)Blood. 117:2451-2459.
警告（S）：避免凍融循環
科學類別：甲基
產品類別：重組蛋白
我們公司zui大優勢是強大的采購，
1：基本什么都能進口，血清，抗體，耗材，還有部分限制進口的，
2：貨品全，現經營過700多個品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產品那就更有優勢，
3：提供加急服務，一般1-2周到貨，超過時限加急費全免
4：價格公道，絕大部分價格有優勢，當然不能保證100%產品都是,因為意味著沒有服務.
5：良好的信譽，大部分客戶我們提供貨到付款服務，客戶包括清華，北大 交大 復旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們*代理的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，QA-Bio,Inc，QA-Bio,IncQuickZyme Biosciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發，歡迎合作。
