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Waters 474 HPLC Scanning Fluorescence Detector

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High levels of sensitivity and specificity make fluorescence an increasing favorable mode of detection for HPLC. Waters 474 Fluorescence detector with advanced programming and scanning capabilities surpasses the previous standard for sensitivity set by the 470 detector.

Product Description

This Waters 474 scanning fluorescence detector has advanced programming and scanning capabilities. The Waters 474 Scanning Fluorescence Detector is a high-performance unit that provides a high level of sensitivity for spectrofluorometric analysis. Features include:  

- Optical design that maximizes sensitivity

- Microprocessor control for greater versatility and reliability

- An emission wavelength sweep function so you can obtain an emission spectrum

- An excitation wavelength sweep function so you can obtain an excitation spectrum

- An automatic second-order filter to enhance applications in the visible wavelength

Range

- Optional optical filters that can be installed on the flow cell for specialized

measurements （not included）

- can be used with quartz flow cell or cuvette cell, permitting measurement of ultraviolet fluorescence

- Nine-stage photomultiplier tube for amplifying the emission radiation

- Photodetector to compensate for excitation fluctuations throughout a scan

There are three critical optical design features in the 474 detector:

- A highly energy-efficient optics system that does not use lenses, minimizing

Reflection loss

- An optics system that uses holographic concave diffraction gratings and aspherical

mirrors to provide highly efficient monochromators in a compact package

- A 150 W Xenon lamp that provides greater light energy for excitation

There are five critical microprocessor-based features:

- Operating parameters that are set using the keypad

- A multi-line liquid crystal display （LCD） that provides information on the status of the 474 detector

- Self-diagnostic messages that are displayed on the LCD

- Digital processing that controls the entire signal processing system, assuring higher reliability and operational stability

- An auto-zero feature that provides a wide range of offset adjustment for the analog output

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