
Annexin V/PI法是目前較為流行的檢測細胞凋亡的方法。AnnexinV是一種鈣依賴性的磷脂結合蛋白,對磷脂酰絲氨酸(phosphatidyl-serine,PS)具有較高的親和性,它可以較為敏感的檢測細胞膜表面的翻轉的PS。將Annexin-V進行熒光素(FITC、PE)標記,以標記的Annexin-V作為熒光探針,利用流式細胞或熒光顯微鏡可檢測細胞凋亡的發生。該方法快速可靠,不需要固定細胞,從而避免了Tunel法因固定而出現的DNA 段丟失,該方法廣泛用于細胞的早期凋亡檢測。
Balb/c 小鼠脾細胞流式染色(Southern biotech cat#10010-02)
產品訂購信息:
名稱 | 貨號 | 規格 |
Annexin V-FITC/PI 試劑盒 | 10010-02 | 100T |
Annexin V-PE/7AAD試劑盒 | 10010-09 | 100T |
注:除以上成品外,試劑盒組分均可單獨訂購,客戶可根據自身需求組裝需要的試劑盒。
名稱 | 貨號 | 規格 |
Annexin V-UNLB | 10039-01 | 0.1 mg |
Annexin V-FITC | 10039-02 | 100T |
Annexin V-BIOT | 10039-08 | 100T |
Annexin V-UNLB | 10040-01 | 0.1 mg |
Annexin V-FITC | 10040-02 | 100T |
Annexin V-BIOT | 10040-08 | 100T |
Annexin V-PE | 10040-09 | 100T |
Annexin V-APC | 10040-11 | 100T |
Annexin V-CY5 | 10040-15 | 100T |
7-Aminoactinomycin D (7-AAD) | 10042-01 | 100T |
Propidium Iodide (PI) | 10044-01 | 100T |
Annexin V Binding Buffer 10X | 10045-01 | 50 mL |
應用文獻:
- Corey Speers,etal. Maternal Embryonic Leucine Zipper Kinase (MELK) as a Novel Mediator and Biomarker of Radioresistance in Human Breast Cancer. Clinical Cancer Research..(應用產品:cat#10010-02).
- Mallikarjun Handigund,etal. Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N acetylcysteine (NAC). Transfusion and Apheresis Science. Published Feb(應用產品: cat#10010-02.)
- G Feng,etal..Interleukin21 mediates hepatitis B virus‐associated liver cirrhosis by activating hepatic stellate cells.(應用產品: cat#10010-09).
- Ruonan Xu,etal. Complement 5a stimulates hepatic stellate cells in vitro, and is increased in the plasma of patients with chronic hepatitis B. Immunology. (應用產品: cat#10010-09)
- Zhu, Jin-Hui,etal.Suppression of Cellular Apoptosis Susceptibility (CSE1L) Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells. Cancer Prevetion. (應用產品: cat#10010-09)
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