28105-052130Thermo賽默飛Hypersil BDS C18色譜柱自 1989 年發明以來，一直廣受業界推崇，被認為是耐用可信賴的色譜柱之一。Hypersil BDS 色譜柱以高度堿性去活硅膠為擔體，采用封端技術屏蔽硅醇基，具有以下優點：

* 出色的重現性

* 明顯改善峰拖尾

* 十分耐用，色譜柱使用壽命較長

* 不論是堿性化合物還是酸性化合物，均呈現wan美峰形。

為什么要用堿性去活硅膠和鍵合相？

使用共價鍵合硅膠固定相，可對多數化合物進行分離。HPLC 與液-液分配色譜相比，具有平衡速度快、分配性能更好的特點，因此，HPLC 作為現代分析手段被廣泛應用。

但是，傳統的共價鍵合硅膠固定相存在以下問題：

HPLC 中使用的建和硅膠固定相與分析物之間存在多種相互作用，這些相互作用一部分是鍵合相本身與分析物之間的作用，另一部分是鍵合時在硅膠表面殘留的硅醇基與分析物之間的作用。

硅醇基的數目和酸性極大程度影響了它與分析物間相互作用的強弱。堿性硅膠的酸-堿相互作用主要是由硅醇基產生的，硅醇基還決定了硅膠填料的表面極性。硅醇基的種類和酸性在很大程度上影響了 HPLC 的分離度和分析物的色譜峰形。

殘余硅醇基與化合物的相互作用會使酸性和堿性化合物的色譜峰產生拖尾。早期使用的 HPLC 硅膠填料，硅醇基具有很強的酸性，因此色譜拖尾現象特別明顯。

使用早期硅膠填料時，若要得到較好的色譜峰，通常需要在流動相中添加酸（醋酸）或堿（三乙胺），使其與硅醇基產生競爭性結合，從而改善色譜峰形。當使用了合適的流動相添加劑時，色譜峰形會得到明顯改善。通常需要很高的添加劑濃度多數情況下達到 1% （體積比）或更高，這樣才能使所有殘留硅醇基都與添加劑結合，而不影響分析物的色譜峰形。但是高濃度添加劑常常嚴重降低色譜柱壽命和方法重現性。

所以Hypersil BDS 是的堿性去活硅膠之一，具有以下優點：

* 減弱硅醇基相互作用

* 改善色譜峰拖尾

* 不需向流動相中加入添加劑

* 的色譜峰對稱性

* 延長色譜柱使用壽命

* 提高柱效（分析堿性、酸性、中性化合物）

Thermo賽默飛Hypersil BDS C18色譜柱

Hypersil BDS重現性，批次間測試流程，多種固定相：
Hypersil BDS 色譜柱是根據最高標準生產的，并進過嚴苛的質量控制。硅膠擔體和色譜柱的生產流程均遵守 ISO9001:2000 標準。在進行鍵合前，BDS 硅膠必須通過 30 余項物理特性和色譜性能測試。在鍵合后，所有的 BDS C18 填料都經過色譜性能測試以及碳載量測試該測試在封端前和封端后各進行一次。

Hypersil BDS 色譜柱有 4 種鍵合相供選擇。所有 Hypersil BDS 色譜柱，均使用堿性去活工藝處理硅膠擔體，再經封端處理屏蔽硅醇基，即使是分析堿性化合物，也極大程度改善了峰拖尾。

Hypersil BDS填料參數

填料顆粒形狀/大小（um）孔徑(?) 表面積(m2/g) 碳載量% 端基封尾 USP PH 范圍

Hypersil BDS C18 Spher. 2.4，3, 5 130 170 11 Yes L1 2-9

Hypersil BDS C8 Spher. 2.4，3, 5 130 170 7 Yes L7 2-9

Hypersil BDS Phenyl Spher. 2.4，3, 5 130 170 5 Yes L11 2-9

Hypersil BDS CN Spher. 2.4，3, 5 130 170 4 Yes L10 2-9

Hypersil BDS供貨信息

貨號描述

28102-012101 10x2.1mm 2.4um Hypersil BDS C18

28102-052130 50x2.1mm 2.4 um Hypersil BDS C18

28102-054630 50x4.6mm 2.4 umHypersil BDS C18

28102-102130 100x2.1mm 2.4 um Hypersil BDS C18

28102-104630 100x4.6mm 2.4 um Hypersil BDS C18

28102-152130 150x2.1mm 2.4 umHypersil BDS C18

28102-154630 150x4.6mm 2.4 umHypersil BDS C18

28103-012101 10x2.1mm 3um BDS C18 保護柱柱芯

28103-013001 10x3mm 3um BDS C18 保護柱柱芯

28103-014001 10x4mm 3um BDS C18 保護柱柱芯

28103-052130 50x2.1mm 3umHypersil BDS C18

28103-053030 50x3mm 3umHypersil BDS C18

28103-054030 50x4mm 3umHypersil BDS C18

28103-054630 50x4.6mm 3um BDS C18

28103-102130 100x2.1mm 3um BDS C18

28103-104030 100x4mm 3um BDS C18

28103-104630 100x4.6mm 3um BDS C18

28103-124030 125x4mm 3u BDS C18

28103-124630 125x4.6mm 3u BDS C18

28103-152130 150x2.1mm 3um BDS C18

28103-154630 150x4.6mm 3um BDS C18

28103-204630 200x4.6mm 3u BDS C18

28103-252130 250x2.1mm 3u Hypersil BDS C18

28103-253030 250x3mm 3u Hypersil BDS C18

28103-254030 250x4mm 3u Hypersil BDS C18

28103-254630 250x4.6mm 3u Hypersil BDS C18

28105-011001 10x1mm 5um Hypersil BDS C18 保護柱柱芯

28105-012101 10x2.1mm 5um Hypersil BDS C18 保護柱柱芯

28105-012106 10x2.1mm 5u BDS C18 Javelin 直連式保護柱

28105-013001 10x3mm 5um BDS C18 保護柱柱芯

28105-014001 10x4mm 5um BDS C18 保護柱柱芯

28105-052130 50x2.1mm 5um Hypersil BDS C18

28105-054630 50x4.6mm 5um Hypersil BDS C18

28105-102130 100x2.1mm 5umHypersil BDS C18

28105-104630 100x4.6mm 5um Hypersil BDS C18

28105-122130 125x2.1mm 5umHypersil BDS C18

28105-124030 125x4mm 5umHypersil BDS C18

28105-124630 125x4.6mm 5umHypersil BDS C18

28105-152130 150x2.1mm 5umHypersil BDS C18

28105-153030 150x3mm 5um Hypersil BDS C18

28105-154030 150x4mm 5umHypersil BDS C18

28105-154630 150x4.6mm 5umHypersil BDS C18

28105-159270 150x21.2mm 5u Hypersil BDS C18 制備柱

28105-202130 200x2.1mm 5umHypersil BDS C18

28105-204030 200x4mm 5umHypersil BDS C18

28105-204630 200x4.6mm 5um Hypersil BDS C18

28105-252130 250x2.1mm 5um Hypersil BDS C18

28105-252168 250x2.1mm 5umHypersil BDS C18 PEEK柱

28105-254030 250x4mm 5um Hypersil BDS C18

28105-254630 250x4.6mm 5um Hypersil BDS C18

28105-259070 250x10mm 5um BDS C18 制備柱

28105-259270 250x21.2mm 5um BDS C18 制備柱

28105-304030 300x4mm 5um BDS C18

28105-304630 300x4.6mm 5um BDS C18

28202-012101 10x2.1mm 2.4um BDS C8

28202-014001 10x4mm 2.4um Hypersil BDS C8

28202-052130 50x2.1mm 2.4 um Hypersil BDS C8

28202-054630 50x4.6mm 2.4 um Hypersil BDS C8

28202-102130 100x2.1mm 2.4 um Hypersil BDS C8

28202-104630 100x4.6mm 2.4 um Hypersil BDS C8

28202-152130 150x2.1mm 2.4 um Hypersil BDS C8

28202-154630 150x4.6mm 2.4 um Hypersil BDS C8

28203-012101 10x2.1mm, 3um Hypersil Hypersil BDS C8 保護柱柱芯

28203-013001 10x3mm, 3um Hypersil Hypersil BDS C8 保護柱柱芯

28203-014001 10x4mm, 3um Hypersil Hypersil BDS C8 保護柱柱芯

28203-032130 30x2.1mm 3u Hypersil BDS C8

28203-033030 30x3mm 3u Hypersil BDS C8

28203-034630 30x4.6mm 3u Hypersil BDS C8

28203-052130 50x2.1mm 3um Hypersil BDS C8

28203-053030 50x3mm 3um Hypersil BDS C8

28203-054030 50x4mm 3um Hypersil BDS C8

28203-054630 50x4.6mm 3um Hypersil BDS C8

28203-074630 75x4.6mm 3u Hypersil BDS C8 column

28203-102130 100x2.1mm 3um Hypersil Hypersil BDS C8

28203-103030 100x3mm 3um Hypersil Hypersil BDS C8

28203-104030 100x4mm 3um Hypersil Hypersil BDS C8

28203-104630 100x4.6mm 3um Hypersil Hypersil BDS C8

28203-123030 125x3mm 3u Hypersil BDS C8

28203-124630 125x4.6mm 3u Hypersil BDS C8

28203-152130 150x2.1mm 3um Hypersil Hypersil BDS C8

28203-153030 150x3mm 3um Hypersil Hypersil BDS C8

28203-154030 150x4mm 3um Hypersil Hypersil BDS C8

28203-154630 150x4.6mm 3um Hypersil Hypersil BDS C8

28203-252130 250x2.1mm 3u Hypersil BDS C8

28203-254630 250x4.6mm 3u Hypersil BDS C8

28205-011001 10x1mm, 5um Hypersil Hypersil BDS C8 保護柱柱芯

28205-012101 10x2.1mm, 5um Hypersil Hypersil BDS C8 保護柱柱芯

28205-032130 30x2.1mm 5u Hypersil BDS C8

28205-034630 30x4.6mm 5u Hypersil BDS C8

28205-052130 50x2.1mm 5um Hypersil BDS C8

28205-053030 50x3mm 5um Hypersil BDS C8

28205-054030 50x4mm 5um Hypersil Hypersil BDS C8

28205-054630 50x4.6mm 5um Hypersil Hypersil BDS C8

28205-102130 100x2.1mm 5um Hypersil Hypersil BDS C8

28205-103030 100x3mm 5um Hypersil Hypersil BDS C8

28205-104030 100x4mm 5um Hypersil Hypersil BDS C8

28205-104630 100x4.6mm 5um Hypersil Hypersil BDS C8

28205-124030 125x4mm 5u Hypersil BDS C8

28205-124630 125x4.6mm 5u Hypersil BDS C8

28205-152130 150x2.1mm 5um Hypersil Hypersil BDS C8

28205-153030 150x3mm 5um Hypersil Hypersil BDS C8

28205-154030 150x4mm 5um Hypersil Hypersil BDS C8

28205-154630 150x4.6mm 5um Hypersil Hypersil BDS C8

28205-159070 150x10mm 5u Hypersil BDS C8 制備柱

28205-204630 200x4.6mm 5u Hypersil BDS C8

28205-252130 250x2.1mm 5um Hypersil Hypersil BDS C8

28205-253030 250x3mm 5um Hypersil Hypersil BDS C8

28205-254030 250x4mm 5um Hypersil Hypersil BDS C8

28205-254630 250x4.6mm 5um Hypersil Hypersil BDS C8

28205-304030 300x4mm 5u Hypersil Hypersil BDS C8

28802-052130 50x2.1mm 2.4 um Hypersil BDS CN

28802-054630 50x4.6mm 2.4 um Hypersil BDS CN

28802-102130 100x2.1mm 2.4 um Hypersil BDS CN

28802-152130 150x2.1mm 2.4 um Hypersil BDS CN

28803-012101 10x2.1mm, 3um Hypersil Hypersil BDS Cyano 保護柱柱芯

28803-052130 50x2.1mm 3um Hypersil Hypersil BDS Cyano

28803-054630 50x4.6mm 3um Hypersil Hypersil BDS Cyano

28803-102130 100x2.1mm 3um Hypersil Hypersil BDS Cyano

28803-104630 100x4.6mm 3um Hypersil Hypersil BDS Cyano

28803-152130 150x2.1mm 3um Hypersil Hypersil BDS Cyano

28803-153030 150x3mm 3um Hypersil Hypersil BDS Cyano

28803-154030 150x4mm 3um Hypersil Hypersil BDS Cyano

28803-154630 150x4.6mm 3um Hypersil Hypersil BDS Cyano

28805-012101 10x2.1mm, 5um Hypersil Hypersil BDS Cyano 保護柱柱芯

28805-014001 10x4mm, 5um Hypersil Hypersil BDS Cyano 保護柱柱芯

28805-052130 50x2.1mm 5um Hypersil Hypersil BDS Cyano

28805-053030 50x3mm 5um Hypersil Hypersil BDS Cyano

28805-054030 50x4mm 5um Hypersil Hypersil BDS Cyano

28805-054630 50x4.6mm 5um Hypersil Hypersil BDS Cyano

28805-102130 100x2.1mm 5um Hypersil Hypersil BDS Cyano

28805-103030 100x3mm 5um Hypersil Hypersil BDS Cyano

28805-104030 100x4mm 5um Hypersil Hypersil BDS Cyano

28805-104630 100x4.6mm 5um Hypersil Hypersil BDS Cyano

28805-152130 150x2.1mm 5um Hypersil Hypersil BDS Cyano

28805-153030 150x3mm 5um Hypersil Hypersil BDS Cyano

28805-154030 150x4mm 5um Hypersil Hypersil BDS Cyano

28805-154630 150x4.6mm 5um Hypersil Hypersil BDS Cyano

28805-204630 200x4.6mm 5u Hypersil BDS CPS

28805-252130 250x2.1mm 5um Hypersil BDS Cyano

28805-254030 250x4mm 5um Hypersil Hypersil BDS Cyano

28805-254630 250x4.6mm 5um Hypersil Hypersil BDS Cyano

28805-304030 300x4mm 5u Hypersil Hypersil BDS CPS

28805-304630 300x4.6mm 5u Hypersil BDS CPS

28902-052130 50x2.1mm 2.4um Hypersil BDS Phenyl

28902-054630 50x4.6mm 2.4um Hypersil BDS Phenyl

28902-102130 100x2.1mm 2.4u Hypersil BDS Phenyl

28902-154630 150x4.6mm 2.4u Hypersil BDS Phenyl

28903-012101 10x2.1mm, 3um Hypersil Hypersil BDS Phenyl 保護柱柱芯

28903-052130 50x2.1mm 3um Hypersil Hypersil BDS Phenyl

28903-054630 50x4.6mm 3um Hypersil Hypersil BDS Phenyl

28903-102130 100x2.1mm 3um Hypersil Hypersil BDS Phenyl

28903-104030 100x4mm 3um Hypersil Hypersil BDS Phenyl

28903-104630 100x4.6mm 3um Hypersil Hypersil BDS Phenyl

28903-152130 150x2.1mm 3um Hypersil Hypersil BDS Phenyl

28903-153030 150x3mm 3um Hypersil Hypersil BDS Phenyl

28903-154030 150x4mm 3um Hypersil Hypersil BDS Phenyl

28903-154630 150x4.6mm 3um Hypersil Hypersil BDS Phenyl

28905-012101 10x2.1mm, 5um Hypersil Hypersil BDS Phenyl 保護柱柱芯

28905-014001 10x4mm, 5um Hypersil Hypersil BDS Phenyl 保護柱柱芯

28905-052130 50x2.1mm 5um Hypersil Hypersil BDS Phenyl

28905-054630 50x4.6mm 5um Hypersil Hypersil BDS Phenyl

28905-102130 100x2.1mm 5um Hypersil Hypersil BDS Phenyl

28905-104030 100x4mm 5um Hypersil Hypersil BDS Phenyl

28905-104630 100x4.6mm 5um Hypersil Hypersil BDS Phenyl

28905-152130 150x2.1mm 5um Hypersil Hypersil BDS Phenyl

28905-154030 150x4mm 5um Hypersil Hypersil BDS Phenyl

28905-154630 150x4.6mm 5um Hypersil Hypersil BDS Phenyl

28905-204630 200x4.6mm 5u Hypersil BDS Phenyl

28905-252130 250x2.1mm 5um BDS Phenyl

28905-254030 250x4mm 5um BDS Phenyl

28905-254630 250x4.6mm 5um BDS Phenyl